Analytical Development
Dowpharma
SM has considerable in-house analytical capabilities to support process and method development and to fully characterize your
protein or peptide, including significant capabilities at our San Diego biotechnology center.
Strain development and fermentation optimization:
- DNA sequencing
- Expression analysis using HTP ELISA, parallel UV-vis or fluorescence detection, real-time PCR, proteomic and microarray technologies
- Online process analytical capabilities for fermentation
- Robotic sample handling and HTP screening capability
Downstream process development:
- HPLC using multiple modes of detection including fluorescence, photodiode array, refractive index, evaporative light scattering, chiral, mass spectrometry, conductivity, and pulsed amperometric
- Gas chromatography (flame ionization and mass spectrometry detection)
- High performance capillary electrophoresis (photodiode array and mass spectrometry detection)
Product characterization:
- Full capability for protein microheterogeneity characterization
- Electrospray ionization mass spectrometry (ESI-MS), matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), and tandem mass spectrometry (MS/MS) for peptide mapping and amino acid sequence analysis
- Fluorescence spectroscopy
- NMR spectrometry
- Assay development
- Bioassay development (mammalian cell culture in vitro assays)
- Total host cell, endotoxin and nucleic acid assays
Dowpharma clients can take advantage of the broad range of capabilities Dow offers at its sites outside our San Diego facility. These capabilities include:
- Nanoelectrospray-LC-MS/MS (Q-TOF)
- MALDI-TOF (with post source decay capability)
- Circular dichroism (CD)
- Fourier transform infrared spectroscopy (FT-IR)
- Electron paramagnetic resonance (EPR)
- Inductively coupled plasma mass spectrometry (ICP-MS)
For more information about how our analytical development capabilities can help you, contact us.
SMService mark of The Dow Chemical Company
